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( a – e ). Immunohistochemical staining <t>for</t> <t>G-CSF</t> in endometrium on cycle days 29 ( a ), 46 ( b ), 15 ( c ), 24 ( d ), and 16 ( e ). G-CSF positivity is detected mainly in stromal and periglandular cells (arrows), with focal epithelial immunoreactivity. Intensity and distribution vary between cycle days. Magnification: 200× ( a , c , d ); 250× ( b , e ). Abbreviations: LE—Luminal Epithelium; GE—Glandular Epithelium; S—Stroma.
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( a – e ). Immunohistochemical staining <t>for</t> <t>G-CSF</t> in endometrium on cycle days 29 ( a ), 46 ( b ), 15 ( c ), 24 ( d ), and 16 ( e ). G-CSF positivity is detected mainly in stromal and periglandular cells (arrows), with focal epithelial immunoreactivity. Intensity and distribution vary between cycle days. Magnification: 200× ( a , c , d ); 250× ( b , e ). Abbreviations: LE—Luminal Epithelium; GE—Glandular Epithelium; S—Stroma.
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( a – e ). Immunohistochemical staining <t>for</t> <t>G-CSF</t> in endometrium on cycle days 29 ( a ), 46 ( b ), 15 ( c ), 24 ( d ), and 16 ( e ). G-CSF positivity is detected mainly in stromal and periglandular cells (arrows), with focal epithelial immunoreactivity. Intensity and distribution vary between cycle days. Magnification: 200× ( a , c , d ); 250× ( b , e ). Abbreviations: LE—Luminal Epithelium; GE—Glandular Epithelium; S—Stroma.
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( a – e ). Immunohistochemical staining <t>for</t> <t>G-CSF</t> in endometrium on cycle days 29 ( a ), 46 ( b ), 15 ( c ), 24 ( d ), and 16 ( e ). G-CSF positivity is detected mainly in stromal and periglandular cells (arrows), with focal epithelial immunoreactivity. Intensity and distribution vary between cycle days. Magnification: 200× ( a , c , d ); 250× ( b , e ). Abbreviations: LE—Luminal Epithelium; GE—Glandular Epithelium; S—Stroma.
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( a – e ). Immunohistochemical staining for G-CSF in endometrium on cycle days 29 ( a ), 46 ( b ), 15 ( c ), 24 ( d ), and 16 ( e ). G-CSF positivity is detected mainly in stromal and periglandular cells (arrows), with focal epithelial immunoreactivity. Intensity and distribution vary between cycle days. Magnification: 200× ( a , c , d ); 250× ( b , e ). Abbreviations: LE—Luminal Epithelium; GE—Glandular Epithelium; S—Stroma.

Journal: Current Issues in Molecular Biology

Article Title: Cycle-Dependent Expression of Immune, Morphogenetic, Apoptotic, and Steroid-Related Markers in the Endometrium of Infertile Women: A Pilot Study

doi: 10.3390/cimb48030264

Figure Lengend Snippet: ( a – e ). Immunohistochemical staining for G-CSF in endometrium on cycle days 29 ( a ), 46 ( b ), 15 ( c ), 24 ( d ), and 16 ( e ). G-CSF positivity is detected mainly in stromal and periglandular cells (arrows), with focal epithelial immunoreactivity. Intensity and distribution vary between cycle days. Magnification: 200× ( a , c , d ); 250× ( b , e ). Abbreviations: LE—Luminal Epithelium; GE—Glandular Epithelium; S—Stroma.

Article Snippet: Five different targets were examined using the following: G-CSF (sc-53292, monoclonal, working dilution 1:100, Santa Cruz biotechnology, Inc., Dallas, TX, USA) [ , ]; BMP-2/4 (AV103, monoclonal, working dilution 1:100, R&D Systems, Bio-Techne, Minneapolis, MI, USA) [ ]; HSP-70 (585054A, monoclonal, working dilution 1:50, Invitrogen, Waltham, MA, USA) [ , ]; Progesterone (IR068, monoclonal, working dilution 1:100, Dako, Carpinteria, CA, USA) [ ]; Estrogen (GA084, monoclonal, working dilution 1:100, Dako) [ , ].

Techniques: Immunohistochemical staining, Staining

Mean positive cell count of Hsp-70, TUNEL, G-CSF and BMP-2/4.

Journal: Current Issues in Molecular Biology

Article Title: Cycle-Dependent Expression of Immune, Morphogenetic, Apoptotic, and Steroid-Related Markers in the Endometrium of Infertile Women: A Pilot Study

doi: 10.3390/cimb48030264

Figure Lengend Snippet: Mean positive cell count of Hsp-70, TUNEL, G-CSF and BMP-2/4.

Article Snippet: Five different targets were examined using the following: G-CSF (sc-53292, monoclonal, working dilution 1:100, Santa Cruz biotechnology, Inc., Dallas, TX, USA) [ , ]; BMP-2/4 (AV103, monoclonal, working dilution 1:100, R&D Systems, Bio-Techne, Minneapolis, MI, USA) [ ]; HSP-70 (585054A, monoclonal, working dilution 1:50, Invitrogen, Waltham, MA, USA) [ , ]; Progesterone (IR068, monoclonal, working dilution 1:100, Dako, Carpinteria, CA, USA) [ ]; Estrogen (GA084, monoclonal, working dilution 1:100, Dako) [ , ].

Techniques: Cell Characterization, TUNEL Assay